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特发性血小板减少性紫癜是由于免疫因素引起,但长期以来缺乏理想的抗体检测方法。本文采用~(125)I标记的葡萄球菌A蛋白(SPA),直接与患者血小板表面的IgG(PA IgG)结合,而后在重力离心的作用下,使血小板穿过比重为1.012的中性油沉积于管底,与反应液分离,测出血小板沉积块所带放射性,从而计算出平均
Idiopathic thrombocytopenic purpura due to immune factors, but the lack of an ideal antibody test for a long time. In this study, 125I-labeled staphylococcal protein A (SPA) was used to directly bind PA IgG on the surface of platelets. After that, platelets were passed through neutral oil with a specific gravity of 1.012 under the action of gravity centrifugation At the bottom of the tube, the reaction mixture was separated and radioactivity in the platelet sediment was measured to calculate the average