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目的 检测植物血凝素及淋巴因子激活的杀伤细胞（ＰＨＡ－ＬＡＫ）的体外广谱抗肿瘤作用。方法 用ＭＴＴ比色法测定 ＰＨＡ－ＬＡＫ对体外培养的Ｋ５６２、ＭＧｃ８０－３、１４３ＴＫ－、ＨｅＬａ、ＬｏＶｏ等５种肿瘤细胞的杀伤活性。结果ＰＨＡ－ＬＡＫ对５种不 同组织器官来源的肿瘤细胞均有明显杀伤活性，在效靶比为７．５：１．０时，ＰＨＡ－ＬＡＫ对１４３ＴＫ－细胞的杀伤率可高达 ５７．３％，杀伤效应随效靶比增加而升高；但在效靶比为１５：１时，杀伤效应不随效靶比增加而增高数。结论 （１）来自健康献血 员的ＰＨＡ－ＬＡＫ对肿瘤细胞具非特异性杀伤活性，能较好解决传统过继免疫治疗中免疫效应细胞的数量和活性问题； （２）在一定的条件下，ＭＴＴ法用于检测免疫效应细胞的杀瘤作用时才具灵敏、可靠的优点。
Objective To investigate the anti-tumor effect of phytohemagglutinin and lymphokine activated killer cells (PHA-LAK) in vitro. Methods The cytotoxicity of PHA-LAK against five kinds of tumor cells such as K562, MGc80-3, 143TK-, HeLa and LoVo was measured by MTT assay. Results PHA-LAK had significant killing activity on tumor cells derived from 5 different tissues and organs. The kill rate of PHA-LAK against 143 TK-cells was up to 57.3% when the target ratio was 7.5:1.0. The killing effect increased with the increase of the effect-target ratio, but at the effect ratio of 15:1, the killing effect did not increase with the increase of the effect-target ratio. Conclusion (1) PHA-LAK from healthy blood donors has non-specific killing activity on tumor cells, which can better solve the problem of the number and activity of immune effector cells in traditional adoptive immunotherapy; (2) under certain conditions, MTT method It is sensitive and reliable when used to detect tumorigenic effects of immune effector cells.