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目的：克隆并分析人血管内皮生长因子（ＶＥＧＦ）单克隆抗体重链可变区基因。方法：从分泌抗人ＶＥＧＦ单抗的杂交瘤细胞株（Ｅｌ）中提取总ＲＮＡ，利用逆转录－ＰＣＲ方法，克隆抗人ＶＥＧＦ单克隆抗体（ＭＡＢ）重链可变区基因（ＶＨ），将其重组入ｐＥＧＭＲ－ＴＶｅｃｔｏｒ测序载体测序。结果：ＶＨ基因序列全长３６９个碱基对，编码１２３个氨基酸，通过国际联机检索及Ｋａｂａｔ库扫描证实，该ＶＨ基因符合小鼠免疫球蛋白可变区基因特征，同源性最高达８７％。结论：ＶＨ基因全长３６９ｂｐ，根据Ｋａｂａｔ分类，归属小鼠重链可变区基因第Ⅱ（Ａ）亚组，由ＶＨ－Ｄ－ＪＨ４重排产生。
Objective: To clone and analyze the variable region gene of human monoclonal antibody of vascular endothelial growth factor (VEGF). METHODS: Total RNA was extracted from the hybridoma cell line (El) secreting anti-human VEGF mAb. The heavy chain variable region (VH) of anti-human VEGF monoclonal antibody (MAB) was cloned by reverse transcription-PCR It was recombined into the pEGMR-TVector sequencing vector. Results: The VH gene was 369 base pairs in length and encoded 123 amino acids. The VH gene conformed to the mouse immunoglobulin variable region gene characteristics by online search and Kabat bank scan. The homology was up to 87% . Conclusion: The VH gene is 369bp in length. According to the Kabat classification, subunit Ⅱ (A) of mouse heavy chain variable region gene is produced by rearrangement of VH-D-JH4.