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研究了两种微藻──小新月菱形藻与青岛大扁藻在各自的抑制剂量范围内(小新月菱形藻0~3.00J/m2;青岛大扁藻0~11.25J/m2)UV-B辐射对其相对增长率(K)、蛋白质含量、类胡萝卜素含量以及超氧化物歧化酶(Superoxidedismutase,SOD)活性的影响。结果表明,(1)随着辐射剂量的升高,两种微藻的相对增长率(K)逐渐下降,其各自的半数有效抑制浓度48h·EC50分别为:小新月菱形藻1.79J/m2;青岛大扁藻7.80J/m2。(2)蛋白质与光合色素含量的变化趋势基本类似于相对增长率的变化。相对增长率是灵敏指示微藻生长变化的优选指标。(3)超氧化物歧化酶(SOD)的活性也受到影响,在其各自的最大实验剂量下小新月菱形藻SOD活性下降21%;青岛大扁藻SOD活性下降17%。
Two species of microalgae, Pseudomonas aeruginosa and Platymonas sp., Were studied in the range of their respective inhibition dose (0-3.00J / m 2 for P. xinnensis and 0-11.25 J / m 2 for P. maculata ) UV-B radiation on its relative growth rate (K), protein content, carotenoid content and superoxide dismutase (SOD) activity. The results showed that: (1) With the increase of radiation dose, the relative growth rate (K) of the two microalgae decreased gradually, and their respective half effective inhibitory concentrations (48h · EC50) were 1.79J / m2; Platycodon grandiflorum 7.80J / m2. (2) The change trend of protein and photosynthetic pigment content is basically similar to the change of relative growth rate. Relative growth rate is a sensitive indicator of microalgae growth and change of the preferred indicators. (3) The activity of superoxide dismutase (SOD) was also affected. Under the respective maximal experimental dose, the SOD activity decreased by 21% and the SOD activity decreased by 17%.