论文部分内容阅读
目的建立人类ether-a-go-go相关基因1b(human ether-a-go-go-related gene 1b,hERG1b)钾通道在爪蟾卵母细胞上的异源性表达方法和hERG1b钾通道电流记录方法,研究hERG1b钾通道的电生理学特性。方法将编码hERG1b亚基的cRNA注入爪蟾卵母细胞中表达hERG1b钾通道,使用双电极电压钳技术记录全细胞电流,分析hERG1b钾通道电流门控动力学特征和电生理特性。结果与结论利用双电极电压钳技术,记录到hERG1b钾电流,该电流具有内向整流特性,胞外钾离子浓度升高可增大hERG1b钾通道电流幅度。与hERG1a钾通道电流相比,hERG1b钾通道电流的去激活速度明显加快,表现出快速去激活的电生理特征。
Objective To establish a heterologous expression profile of human ether-a-go-go gene 1b (potassium channel) on Xenopus oocytes and hERG1b potassium channel current recording Methods to study the electrophysiological properties of hERG1b potassium channels. Methods cRNA encoding hERG1b subunit was injected into Xenopus laevis oocytes to express hERG1b potassium channels. Whole-cell currents were recorded by two-electrode voltage-clamp technique. The kinetic characteristics and electrophysiological properties of hERG1b potassium channel current gating were analyzed. RESULTS AND CONCLUSION: The hERG1b potassium current was recorded using the bipolar voltage clamp technique. This current has inward rectification characteristics. Increasing extracellular potassium concentration can increase the current amplitude of hERG1b potassium channel. Compared with the hERG1a potassium channel current, the deactivation of hERG1b potassium channel currents was significantly accelerated, showing electrophysiological characteristics of rapid deactivation.