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为将 Vit K3(menadion)增强的 MTT(四甲基偶氮唑盐 )方法 (menadion- augmented MTT method)用于分析白念珠菌细胞的活力，采用正交设计 [L16(4321)]确定其最优基本反应条件，用该方法分析不同菌浓度的白念珠菌以及其在受到不同化学物质作用后的活力变化，并将该方法用于其它病原性酵母菌及酵母样真菌。实验结果表明 menadion增强的 MTT方法最优基本反应条件为： MTT 1.2g/L、 menadion 20mmol/L、反应时间 6h、分光光度计波长为 570nm。该方法可敏感地定量反映出白念珠菌的活力变化，并且能有效的分析其他病原性酵母菌及酵母样真菌的细胞活力。结果提示优化的 MTT方法可以快速、简便、灵敏和定量地分析白念珠菌及某些致病性酵母菌和酵母样真菌的活力。
For the purpose of analyzing the viability of Candida albicans cells using the VitK3 (menadion) enhanced MTT method, the orthogonal design [L16 (4321)] was used to determine the most significant Excellent basic reaction conditions, the method of analysis of different concentrations of Candida albicans and its activity by different chemicals after the change, and the method for other pathogenic yeasts and yeast-like fungi. The experimental results show that the best basic reaction conditions for the enhanced MTT method of menadion are: MTT 1.2g / L, menadion 20mmol / L, reaction time 6h, spectrophotometer wavelength 570nm. The method can sensitively and quantitatively reflect the change of the viability of Candida albicans and can effectively analyze the cell viability of other pathogenic yeasts and yeast-like fungi. The results suggest that the optimized MTT method can quickly and easily, sensitively and quantitatively analyze the activity of Candida albicans and some pathogenic yeasts and yeast-like fungi.