论文部分内容阅读
目的通过观察红花注射液(ICT)对2,4,6-三硝基苯磺酸(2,4,6-TNBS)诱导的大鼠溃疡性结肠炎(UC)的疗效及其对结肠黏膜中粘蛋白(MUC2)、白细胞介素6(IL-6)表达的影响,探讨其作用机制。方法将30只健康雄性Wistar大鼠随机分为对照组10只、模型组10只与红花组10只。采用TNBS灌肠法复制大鼠UC模型,红花组同时给予ICT干预治疗,模型组与对照组给予等量生理盐水。造模10d后,对大鼠行疾病活动指数(DAI)、结肠大体形态评分及组织学损伤评分,分别用免疫组织化学法及实时荧光定量PCR(RT-PCR)技术观察大鼠结肠黏膜中MUC2,IL-6的表达。结果与对照组相比,实验组与模型组DAI评分、结肠大体形态评分、组织学损伤评分均升高,而实验组比模型组显著改善(P<0.01);与对照组相比,模型组MUC2表达显著降低,而红花组又比模型组显著升高(P<0.05)。模型组IL-6表达显著升高,而红花组又比模型组显著降低(P<0.05)。结论 ICT对UC大鼠疗效显著,其作用机制之一可能是通过上调结肠黏膜MUC2及下调IL-6的表达,增强肠黏膜屏障作用.减轻类症反应。
Objective To observe the effects of safflower injection (ICT) on 2,4,6-trinitrobenzene sulfonic acid (2,4,6-TNBS) -induced ulcerative colitis (UC) and its effect on colonic mucosa Mucin (MUC2), interleukin 6 (IL-6) expression, to explore the mechanism of action. Methods Thirty healthy male Wistar rats were randomly divided into control group (n = 10), model group (n = 10) and safflower group (n = 10). TNBS enema was used to replicate the UC model in rats. At the same time, the safflower group was treated with ICT intervention. The model group and the control group were given the same amount of normal saline. After modeling for 10 days, the disease activity index (DAI), gross morphology of colon and histological damage score of rats were evaluated. Immunohistochemistry and real-time quantitative PCR (RT-PCR) were used to observe the expression of MUC2 , IL-6 expression. Results Compared with the control group, DAI score, gross morphology score and histological damage score of experimental group and model group were significantly increased, while the experimental group was significantly improved than the model group (P <0.01); compared with the control group, MUC2 expression was significantly decreased, while the safflower group was significantly higher than the model group (P <0.05). The expression of IL-6 in the model group was significantly higher than that in the model group (P <0.05). Conclusion ICT has a significant therapeutic effect on UC rats. One of the possible mechanisms is that ICT can increase the barrier function of intestinal mucosa by up-regulating the expression of MUC2 and down-regulating the expression of IL-6 in colonic mucosa.