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目的:通过对兵团部分地区手足口病病例标本的核酸检测结果进行分析,了解这些地区手足口病的病原学流行情况,为手足口病的预防控制提供参考依据。方法:采用荧光定量RT-PCR方法,对2014年我实验室收到标本进行检测,并对部分标本的VP1序列进行扩增测序,确定其基因型及突变情况。结果:150例被检患儿的粪便标本中有136例肠道通用型病毒阳性,核酸检测阳性率为90.67%。其中EV71型阳性者25例,占18.38%,Cox A16型阳性者95例,占69.85%,其他肠道病毒阳性者16例,占11.76%,其中EV71型的基因型为C4a,Cox A16型的基因型为B1b。结论:兵团这些地区的手足口病的发生有明确的季节与年龄特征,病原学分型也是以EV71型和Cox A16型为主,基因型分别为C4a和B1b,与全国其他地区的分子流行病学特征一致。
OBJECTIVE: To understand the prevalence of HFMD in these areas by analyzing the results of nucleic acid detection of HFMD cases in some areas of the Corps, and to provide a reference for the prevention and control of HFMD. Methods: Fluorescent quantitative RT-PCR was used to detect the samples received in our laboratory in 2014. The VP1 sequences of some samples were amplified and sequenced to determine their genotypes and mutations. Results: There were 136 cases of enterovirus positive in 150 samples of stool samples, the positive rate of nucleic acid detection was 90.67%. Among them, 25 cases were positive for EV71, accounting for 18.38%, 95 cases were positive for Cox A16, accounting for 69.85%, and 16 cases were positive for other enterovirus (11.76%). The EV71 genotype was C4a and Cox A16 Genotype B1b. Conclusion: The incidence of hand, foot and mouth disease in these regions of the Corps is characterized by seasonal and age characteristics. The etiological types are also EV71 and Cox A16, and the genotypes are C4a and B1b, respectively, which are in line with the molecular epidemiology of other parts of the country Consistent features.