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本文介绍浸泡法制备基于滤纸的SERS基底,并分析滤纸SERS基底表面银纳米粒子(AgNP)的分布与浸泡时间的关系。以精浆为检测对象,相比于514nm波长激发,785nm激发可获得更好的光谱数据,同时还比较了该波长激发下精浆的常规拉曼光谱与SERS光谱。更为重要的是,通过采用精浆中654cm-1谱峰强度评估不同浸泡时间下(6h,12h,24h)滤纸SERS基底的增强性能和测量结果的重复性。实验结果表明,12h浸泡获得的滤纸SERS基底表面具有均匀的AgNP分布,在785nm波长激发下,纸基SERS基底可提供增强效果及光谱重复性俱佳的精浆SERS光谱。
This paper introduces the preparation of SERS substrates based on filter paper by immersion method and analyzes the relationship between the distribution of silver nanoparticles (AgNPs) and the soaking time on the surface of filter paper SERS. Using seminal plasma as the detection target, compared with excitation at 514 nm, better spectral data were obtained at 785 nm excitation, and the conventional Raman and SERS spectra of seminal plasma excited by this wavelength were also compared. More importantly, the enhanced performance and repeatability of SERS substrates were evaluated at different immersion times (6h, 12h, 24h) by using 654cm-1 peak intensities in the seminal plasma. The experimental results show that the SERS substrate surface of the filter paper obtained after 12h soaking has a uniform AgNP distribution, and the SERS substrate of the paper-based SERS substrate can provide enhanced SERS spectra with good spectral reproducibility under the excitation of the wavelength of 785nm.