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目的:研究西藏红雪茶多糖含量测定的方法。方法:采用苯酚-硫酸法测定总糖含量,3,5-二硝基水杨酸(DNS)法测定还原糖含量,两者的差值即为多糖含量。结果:总糖标准曲线方程为OD=0.051 4C-0.0146(r=0.999 2),还原糖标准曲线方程为OD=0.022 9C-0.086(r=0.999 9),两者分别在2.5～12.5,8～40 mg.L-1有良好的线性关系。测得LC,LCA,LCP多糖含量分别为63.6%,82.6%,57.6%。结论:该方法操作快速、简便、灵敏、准确、重复性好。
Objective: To study the method of determining the content of polysaccharide in Tibet red tea. Methods: The content of total sugar was determined by phenol-sulfuric acid method, and the content of reducing sugar was determined by DNS method. The difference between the two was polysaccharide content. RESULTS: The standard curve of total sugar was OD = 0.051 4C-0.0146 (r = 0.999 2), the standard curve of reducing sugar was OD = 0.022 9C-0.086 (r = 0.999 9) 40 mg.L-1 has a good linear relationship. The contents of LC, LCA and LCP were 63.6%, 82.6% and 57.6% respectively. Conclusion: The method is rapid, simple, sensitive, accurate and reproducible.