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目的:探讨人工虫草复合液对大鼠离体肾缺血再灌注损伤的保护效应及其作用机制。方法:将72只雄性SD大鼠离体肾脏随机分为生理盐水灌注组、高渗枸橼酸盐腺嘌呤液(HCA液)灌注组和虫草复合液灌注组,分别观察灌注后6h、12h、24h肾损伤程度、肾小管上皮细胞凋亡指数(AI)、肾组织匀浆中超氧化物歧化酶(SOD)活力与丙二醛(MDA)含量。结果:(1)在灌注后6h,与生理盐水组和HCA液组比较,虫草复合液组肾损伤程度较轻;灌注后12h,与生理盐水组比较,HCA液和虫草复合液组肾损伤程度较轻,而HCA液和虫草复合液组肾损伤程度无差异;在灌注后24h,各组肾损伤程度无差异;(2)与HCA液和生理盐水组比较,虫草复合液组在灌注后6h、12h后AI较低(P<0.05)、肾组织匀浆中SOD活力较高(P<0.05)、MDA含量较低(P<0.05);灌注后24hAI、肾组织匀浆中SOD活力与MDA含量无统计学差异(P>0.05);(3)AI与肾组织匀浆中超氧化物歧化酶(SOD)活力负相关(r=-0.724),与丙二醛(MDA)含量正相关(r=0.681)。结论:人工虫草提取液灌注对大鼠离体肾缺血再灌注损伤具有一定的保护性效应,其作用机制可能与抗氧自由基、抗脂质过氧化作用有关。
Objective: To investigate the protective effect of artificial Cordyceps sinensis compound solution on isolated rat renal ischemia-reperfusion injury and its mechanism. METHODS: Isolated kidneys of 72 male SD rats were randomly divided into normal saline injection group, hypertonic citrate adenine solution (HCA solution) perfusion group, and Cordyceps sinensis compound perfusate group. The perfusion was observed at 6 h and 12 h, respectively. The degree of renal injury, apoptotic index (AI) of renal tubular epithelial cells, activity of superoxide dismutase (SOD) and content of malondialdehyde (MDA) in kidney homogenate were measured after 24 hours. RESULTS: (1) Compared with saline group and HCA solution group, the degree of renal damage was milder in the Cordyceps militaris group than in the saline group and the degree of renal damage in the HCA solution and the Cordyceps sinensis complex group was greater than that in the saline group at 12h after infusion. Lighter, but there was no difference in degree of renal injury between HCA solution and Cordyceps sinensis compound solution group; at 24h after perfusion, there was no difference in degree of renal injury between groups; (2)Compared with HCA solution and saline solution group, Cordyceps sinensis compound solution group was 6h after perfusion. After 12 h, the AI was lower (P<0.05), the SOD activity in the kidney homogenate was higher (P<0.05), and the MDA content was lower (P<0.05); the SOD activity and MDA in the kidney homogenate were 24 h after infusion. There was no significant difference in the content (P>0.05); (3) AI was negatively correlated with the activity of superoxide dismutase (SOD) in the kidney homogenate (r=-0.724), and positively correlated with malondialdehyde (MDA) content (r = 0.681). Conclusion: The perfusion of artificial Cordyceps extract has protective effects on rat kidney ischemia-reperfusion injury in vitro. Its mechanism may be related to antioxidant free radicals and anti-lipid peroxidation.