论文部分内容阅读
目的 在对复方红芪提取液的研究中 ,发现其能促进外周神经损伤的修复 ,并发现其有促进许旺细胞增殖的作用。为了进一步探索复方红芪提取液对神经修复影响的机制 ,观察其对许旺细胞中的酪氨酸蛋白激酶 (PTKs)活性的影响。 方法 使用按 1∶160 0 ,1∶32 0 0 ,1∶64 0 0 ,1∶12 80 0稀释的复方红芪提取液 ,培养新鲜切取的SD大鼠坐骨神经。于培养后第 2 4、48和 96h时 ,使用 [Iγ 32 P]ATP掺入、液闪计数法 ,观察许旺细胞中PTKs的活性变化。使用神经生长因子 (NGF)作阳性对照 ,正常培养液作阴性对照。 结果 正常培养条件下 ,离体神经中许旺细胞的PTKs呈无活性状态。神经生长因子组可见PTKs轻度升高 ,至 96h活性仍有活性且较前稍强 ,峰值为 0 15 48。红芪组可见PTKs活性显著增加 ,随后即下降。峰值出现在第 48h ,1∶160 0 ,1∶32 0 0和 1∶64 0 0三种浓度间变化不明显 ,峰值分别为0 44 60、0 332 6和 0 4169,1∶12 80 0浓度时PTKs活性无变化。秩和检验第 48h、1∶64 0 0以上浓度的红芪培养与神经生长因子及正常对照组差异有显著性 (P <0 0 0 5 ) ,以后各时间间差异则无统计学意义。不同浓度的红芪提取液培养不同时间 ,PTKs活性呈负相关变化 ,秩和检验差异有显著性 (P <0 0 0 5 )。 结论 复方红?
OBJECTIVE To study the compound extract of Radix Hedysari and find out that it can promote the repair of peripheral nerve injury and find its role in promoting the proliferation of Schwann cells. In order to further explore the mechanism of Compound Radix Astragali extract on nerve repair and observe its effect on the activity of tyrosine protein kinases (PTKs) in Schwann cells. Methods Fresh sciatic nerve of SD rat was cultured by using the extract of Radix Hedysari diluted 1:160 0, 1:32 0 0, 1:64 0 0, 1:12 80 0. At the 2nd, 4th, 48th and 96th hour after culture, [Iγ 32 P] ATP incorporation and liquid scintillation counting were used to observe the changes of PTKs activity in Schwann cells. NGF was used as a positive control, and normal culture fluid was used as a negative control. Results Under normal culture conditions, the PTKs of Schwann cells in isolated nerve were inactive. In the group of NGF, the PTKs slightly increased, and the activity was still active at 96h, slightly stronger than before, with a peak of 0 15 48. Red Qi group visible PTKs activity increased significantly, then decreased. There was no significant change in peak value between the three concentrations at 48h, 1:160 0, 1:32 0 0 and 1:64 0 0 with peak values of 0 44 60,0 332 6 and 0 4169,1:12 80 0 respectively No change in PTKs activity. There was a significant difference between NGF and normal control group (P <0 05) at 48h after the rank sum test. There was no significant difference in the later time between the two groups. Different concentrations of extracts of Radix extract at different times, PTKs activity was negatively correlated, rank sum test was significant (P <0 0 0 5). Conclusion compound red?