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目的了解HIV抗体检测产生不确定结果的特点及随访情况。方法对绍兴市2007—2013年HIV抗体确证检测为不确定结果样品的来源、复检结果及蛋白印迹试验(WB)带型特点进行分析并跟踪随访。结果 955份复检阳性标本经WB试验报告结果为不确定29份,占3.03%。对29份标本的人作追踪随访,完成随访16人,复检ELISA和RT结果均为阳性的8人随访后均转为阳性;复检单项阳性的8人随访后均转为阴性。29份不确定结果中共检测到6种带型,gp160(+)、p24(+)和gp160(+)p24(+)3种带型最为常见,占89.65%。结论复检结果双阳性的样品阳转率较高,应加强随访并寻求替代确认策略,以减少非特异性反应导致假阳性结果。
Objective To understand the characteristics and follow-up of uncertainties in HIV antibody testing. Methods The source of HIV positive samples from 2007 to 2013 in Shaoxing was determined as the source of indeterminate results, the results of retest and the banding patterns of Western blotting (WB) were analyzed and followed up. Results 955 retest positive samples by WB test results for the uncertainty of 29, accounting for 3.03%. Follow-up was performed on 29 specimens of all the patients, 16 follow-up visits were completed, 8 of them were positive after re-ELISA and RT were all positive, and 8 of them were negative after retesting. There were 6 bands detected in 29 uncertainties. The most common bands were gp160 (+), p24 (+) and gp160 (+) p24 (+), accounting for 89.65%. Conclusion The results of double-positive retest double positive samples higher positive rate, follow-up should be strengthened and alternative confirmation strategies to reduce non-specific reactions lead to false-positive results.