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目的探讨cyclin D1蛋白在子宫内膜癌组织中的表达及其临床意义。方法采用免疫组织化学SP法检测20例正常子宫内膜、38例不典型增生子宫内膜及126例子宫内膜癌组织中cyclin D1蛋白的表达,分析其与子宫内膜癌临床病理因素的关系及对生存率的影响。结果 cyclin D1蛋白表达阳性率及表达强度在正常子宫内膜、不典型增生子宫内膜及子宫内膜癌中呈明显上升趋势,三者之间cyclin D1蛋白表达存在统计学差异(P<0.001)。cyclin D1蛋白表达阳性率及表达强度在Ⅲ～Ⅳ期病例明显高于Ⅰ～Ⅱ期(P=0.006);cyclin D1蛋白在组织学分级G1、G2、G3中的表达逐渐增加(P=0.010);淋巴结转移阳性者明显高于阴性者(P=0.008);Ⅱ型子宫内膜癌明显高于Ⅰ型(P=0.020)。cyclin D1蛋白表达与肌层浸润程度、腹腔液细胞学及患者年龄均无统计学相关(P>0.05)。cyclin D1蛋白阳性组患者的总体生存率明显低于阴性组患者(P=0.017)。结论 cyclin D1蛋白的过表达参与了子宫内膜癌的发生发展,提示其可作为有效的预后因子。
Objective To investigate the expression of cyclin D1 protein in endometrial carcinoma and its clinical significance. Methods The expression of cyclin D1 protein in 20 cases of normal endometrium, 38 cases of atypical hyperplasia endometrium and 126 cases of endometrial carcinoma was detected by immunohistochemical SP method. The relationship between the expression of cyclin D1 and the clinicopathological parameters of endometrial carcinoma And the impact on survival. Results The positive expression rate of cyclin D1 protein and its expression intensity in normal endometrium, atypical hyperplasia endometrium and endometrial carcinoma showed a significant upward trend. There was a significant difference in the expression of cyclin D1 between the three groups (P <0.001) . The positive expression rate of cyclin D1 and its expression in stage Ⅲ ~ Ⅳ were significantly higher than those in stage Ⅰ ~ Ⅱ (P = 0.006). The expression of cyclin D1 in histological grade G1, G2 and G3 increased gradually (P = 0.010) ; Lymph node metastasis was significantly higher than the negative (P = 0.008); type Ⅱ endometrial cancer was significantly higher than the type Ⅰ (P = 0.020). There was no significant correlation between the expression of cyclin D1 and the degree of myometrial invasion, ascitic fluid cytology and patient’s age (P> 0.05). The overall survival rate of patients with positive cyclin D1 protein was significantly lower than that of negative patients (P = 0.017). Conclusion The overexpression of cyclin D1 is involved in the development of endometrial carcinoma, suggesting that it may be an effective prognostic factor.