Objective: The aim of the study was to investigate the ef ect of SGI-1776 combination with DDP in sub-toxic concentration on induction of apoptosis of human ovarian cancer HO-8910 cel s in vitro and to unravel the associated mecha-nisms. Methods: Human ovarian cancer HO-8910 cel s were cultured in vitro. The inhibitory ef ect of SGI-1776 combination with DDP in sub-toxic concentration on induction on viability of human ovarian cancer HO-8910 cel s was evaluated by the MTT assay. Cel apoptosis rate was analyzed by flow cytometry. The proteins expression level related to apoptosis were analyzed by Western blot. Results: SGI-1776 combination with DDP in sub-toxic concentration significantly inhibited the proliferation of human ovarian cancer HO-8910 cel s, and proliferation inhibition rate was increased drastical y compared with normal saline (NS) group or DDP group in sub-toxic concentration or SGI-1776 group in sub-toxic concentration (P﹤0.01). Apoptosis rate markedly increased after the treatment of SGI-1776 combination with DDP in sub-toxic concentration for 48 h. Western blot showed that the expression of bcl-2 protein was down-regulated and protein level of Bax and Cyto-c were depressed by SGI-1776 combination with DDP in sub-toxic concentration. Conclusion: SGI-1776 combination with DDP in sub-toxic concentration could inhibit the cel proliferation and lead to cel apoptosis inhuman ovarian cancer HO-8910 cel s, and its mechanism may be related to through mitochondrial apoptotic pathway.