论文部分内容阅读
目的:建立同时测定民族药红药中苯乙醇苷类化合物plantainoside D和毛蕊花糖苷含量的方法。方法:采用反相高效液相色谱法,用Agilent C18(4.6 mm×250 mm,5μm)柱,以乙腈-1%醋酸溶液(16∶84)为流动相,流速1.0 mL.min-1,检测波长332 nm,柱温30℃。结果:RP-HPLC测定的plantainoside D和毛蕊花糖苷线性范围分别在6.250~100.0 mg.L-1(r=0.999 8)和12.50~500.0 mg.L-1(r=0.999 8),平均回收率分别为101.3%,100.8%,RSD分别为2.6%,2.2%(n=9)。结论:方法简便,精密度、重复性良好,结果准确可靠,可以作为红药药材及相关制剂质量控制的一个有效方法。
Objective: To establish a method for the simultaneous determination of phenylethanoid glycosides plantainoside D and verbascoside in ethnic medicine red drugs. METHODS: A reversed-phase high performance liquid chromatography was used on an Agilent C18 (4.6 mm×250 mm, 5 μm) column with acetonitrile-1% acetic acid solution (16:84) as the mobile phase and a flow rate of 1.0 mL.min-1. Wavelength 332 nm, column temperature 30°C. RESULTS: The linear ranges of plantainoside D and verbascoside determined by RP-HPLC were 6.250-100.0 mg.L-1 (r=0.999 8) and 12.50-500.0 mg.L-1 (r=0.999 8), respectively. It was 101.3% and 100.8%, RSD was 2.6%, and 2.2% (n=9). Conclusion: The method is simple, accurate and reproducible, and the result is accurate and reliable. It can be used as an effective method for quality control of red medicinal herbs and related preparations.