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目的 探讨非小细胞肺癌 (NSCLC)淋巴结微转移的基因诊断方法 ,并分析CK19mRNA、MUC1mRNA作为肺癌微转移检测分子标志物的可行性。方法 应用巢式逆转录聚合酶链反应 (nestedRT PCR) ,对 3 1例NSCLC的 119枚淋巴结中的粘蛋白 1(MUC1)mRNA、角蛋白 19(CK19)mRNA表达情况进行检测 ;对照组为 10例肺良性病变患者的 3 5枚淋巴结。结果 3 1例NSCLC患者的 119枚淋巴结中 ,66枚 ( 5 5 .5 %)淋巴结存在CK19mRNA阳性表达 ,65枚 ( 5 4.5 %)存在MUC1mRNA阳性表达 ;肺良性病变患者 3 5枚淋巴结中CK19mRNA和MUC1mRNA表达均为阴性 ,与肺癌组比较均有显著性差异。结论 MUC1、CK19基因均可作为RT PCR法检测NSCLC患者淋巴结微转移的分子标志物 ,两者联合检测可能有助于早期诊断肺癌转移。
Objective To investigate the gene diagnosis of lymph node micrometastasis in non-small cell lung cancer (NSCLC) and to analyze the feasibility of using CK19mRNA and MUC1mRNA as molecular markers for the detection of micrometastasis in lung cancer. Methods The expressions of mucin 1 (MUC1) mRNA and CK19 mRNA in 119 lymph nodes of 31 cases of NSCLC were detected by nested RT PCR. The control group was 10 35 cases of benign lung disease in patients with lymph nodes. Results Thirty-six (55.5%) of the 119 lymph nodes in 1 NSCLC patients had positive expression of CK19mRNA, 65 (5.55%) had positive expression of MUC1mRNA, CK19mRNA in 35 lymph node benign lesions MUC1mRNA expression were negative, compared with lung cancer group were significantly different. Conclusion Both MUC1 and CK19 genes can be used as molecular markers for micrometastasis of lymph nodes in NSCLC patients by RT-PCR. The combined detection of MUC1 and CK19 may be helpful for the early diagnosis of lung cancer metastasis.