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利用Asominori×IR2 4的染色体片段置换系 (CSSLs)群体 ,对稻米粒长、粒宽和长宽比进行连续两年及 4个地点的QTL表达稳定性分析。结果表明 :3个性状“两年四点”的表现型都为连续分布 ,均存在超亲遗传类型 ;共检测到 13个粒型相关QTL ,其中在 8个环境中都能被重复检测到的QTL有 6个 ,即影响粒长的 qGL 3、控制粒宽的qGW 5a和 qGW 5b以及共同作用于长宽比的qLWR 3、qLWR 5a和 qLWR 5b。这 6个QTL对应置换系的相应性状与背景亲本Asominori的表现型差异在 8个环境中都达到极显著水平 (P <0 0 0 1) ,且同一QTL对应置换系相应性状的表现型在不同环境间呈显著正相关 (r≥ 0 75 ,r0 0 5=0 6 6 6 ) ,说明这 6个QTL表达稳定性较高。由于 qGL 3和 qLWR 3均位于R19 C16 77标记区间 ,qGW 5a和 qLWR 5a位于C2 6 3标记附近 ,qGW 5b和 qLWR 5b被定位在R5 6 9标记附近 ,因此R19、C16 77、C2 6 3和R5 6 9这 4个RFLP标记对优良水稻外观品质的标记辅助选择 (MAS)育种有着重要作用
The stability of QTLs for two consecutive years and four sites in grain length, grain width and aspect ratio of rice was analyzed by using the chromosome segment replacement (CSSLs) population of Asominori × IR2 4. The results showed that the phenotypes of three traits at four years of two years were all continuously distributed, all of which had super-ancestral inheritance types. Thirteen grain-related QTLs were detected, of which eight were detected repeatedly in eight environments There are 6 QTLs, namely qGL 3 affecting grain length, qGW 5a and qGW 5b controlling grain width, and qLWR 3, qLWR 5a and qLWR 5b acting together in aspect ratio. The phenotypic differences between the corresponding traits of these 6 QTLs and the background parent Asominori reached extremely significant levels (P <0.01 01) in 8 environments, and the phenotypes of the corresponding traits of the same QTLs were different There was a significant positive correlation between the environment (r ≥ 0 75, r 0 0 5 = 0 6 6 6), indicating that the six QTLs are highly stable. Since both qGL3 and qLWR3 are located in the R19 C16 77 labeling interval, qGW 5a and qLWR 5a are located near the C2 6 3 marker, and qGW 5b and qLWR 5b are located near the R5 6 9 marker, R19, C16 77, C2 6 and R5 6 9 These 4 RFLP markers play an important role in marker-assisted selection (MAS) breeding for good rice appearance quality