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目的探讨体外培养的Müller细胞在糖基化终末产物(AGEs)作用下增殖活性的变化,以及这种改变对牛视网膜血管内皮细胞(BREC)紧密连接蛋白(occludin)表达的影响。方法培养新生大鼠Müller细胞和BREC,两类细胞均用特异性抗体进行免疫鉴定。将培养的BREC分4组观察:第1组未添加任何细胞上清液;第2组添加正常Müller细胞上清液;第3组添加糖基化终末产物(AGEs)作用后的Müller细胞上清液;第4组无细胞组,为空白对照组。酶联免疫吸附法(ELISA)测定4组细胞上清液中紧密连接蛋白的含量,比较其变化。结果添加正常Müller细胞上清液组紧密连接蛋白表达量最多,未添加任何细胞上清液组次之,添加AGEs作用后的Müller细胞上清液组更少。结论AGEs能促进Müller细胞异常增殖、抑制BREC表达紧密连接蛋白。
Objective To investigate the changes of proliferative activity of cultured Müller cells under the action of advanced glycation end products (AGEs) and the effect of such changes on the expression of occludin in bovine retinal vascular endothelial cells (BRECs). Methods Cultured neonatal rat Müller cells and BREC cells were identified by specific antibodies. The cultured BRECs were divided into 4 groups: no supernatant was added in group 1; supernatant of normal Müller cells was added in group 2; Müller cells treated with glycosylation end products (AGEs) in group 3 Serum; group 4 cell-free group, a blank control group. Enzyme-linked immunosorbent assay (ELISA) determination of four groups of cell supernatant in the content of tight junction protein, compare the changes. Results The expression of Claudin in the supernatant of normal Müller cells was the highest, followed by the supernatant without any supernatant, followed by the addition of AGEs. Conclusion AGEs can promote the abnormal proliferation of Müller cells and inhibit the expression of tight junction protein in BREC.