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以苹果树腐烂病病菌(Valsa mali Miyabe et Yamada)、苹果轮纹病病菌(Botryospuaeria berengeriana)、苹果斑点落叶病病菌(Alternaria alternata)为研究对象,采用生长速率法对侧柏、五倍子、藿香、百部和旋覆花5种植物提取物的抑菌活性进行了测定,并测定了5种植物提取物对3种病原菌中3种细胞壁降解酶活力的影响。结果表明,5种植物提取物对3种病原菌菌丝生长均有不同程度的抑制作用,其中侧柏对3种病原菌的抑制活性最高,抑菌率分别为74.34%、62.13%、65.36%,其次为五倍子和藿香;5种植物提取物对3种病原菌纤维素酶(Cx)、果胶甲基半乳糖醛酸酶(PMG)、多聚半乳糖醛酸酶(PG)都有一定的抑制作用。结合5种植物提取物对3种病原菌菌丝生长及病原菌细胞壁降解酶的抑制作用,侧柏可作为防治这3种病害进一步开发的植物源药剂。
Valsa mali Miyabe et Yamada, Botryospuaeria berengeriana and Alternaria alternata were used as experimental materials to study the effects of growth rate on the growth of Arborvitae, Chinese nutgall, Agastache rugosa, Bacteriostatic and Inula halophila were determined, and the effects of five plant extracts on the activity of three kinds of cell wall degrading enzymes in three kinds of pathogens were tested. The results showed that all the five plant extracts had different degrees of inhibition on the mycelial growth of the three pathogenic bacteria. Among them, Platycladus orientalis had the highest inhibitory activity against three pathogenic bacteria, with antibacterial rates of 74.34%, 62.13% and 65.36%, respectively Gallnut and gallnut; five kinds of plant extracts on the three pathogenic bacteria Cellulose (Cx), pectin methyl galacturonase (PMG), polygalacturonase (PG) have a certain degree of inhibition effect. Combining the inhibitory effects of five plant extracts on the mycelial growth of three pathogens and the cell-wall degrading enzymes of pathogenic bacteria, Arborvitae could be used as a plant-derived agent for the further development of these three diseases.