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采用烟草花叶病毒侵染辣椒植株,用不同浓度水杨酸处理感病的辣椒植株,测定叶绿素、丙二醛等生理指标,分析其生理生化变化。结果表明:烟草花叶病毒侵染后辣椒叶片叶绿素含量降低、丙二醛含量升高、脯氨酸含量下降、过氧化物酶活性和超氧化物歧化酶活力值明显下降,100Lmol/L水杨酸处理后生理特性的变化影响较显著,叶绿素含量升高、丙二醛含量降低、脯氨酸含量升高、过氧化物酶和超氧化物歧化酶活性升高,而500Lmol/L的水杨酸对辣椒形成胁迫效应。表明外源水杨酸(<500Lmol/L)可以显著提高感染TM V辣椒的抗病性,有利于辣椒幼苗对逆境的适应。
Tobacco plants were infected with tobacco mosaic virus, the susceptible pepper plants were treated with different concentrations of salicylic acid, and the physiological indexes such as chlorophyll and malondialdehyde were measured and their physiological and biochemical changes were analyzed. The results showed that after tobacco mosaic virus infection, the content of chlorophyll decreased, the content of malondialdehyde increased, the content of proline decreased, the activity of peroxidase and the activity of superoxide dismutase decreased markedly, while 100Lmol / L salicylide Physiological characteristics changed more significantly after acid treatment. Chlorophyll content, MDA content and proline content increased, and peroxidase and superoxide dismutase activities increased. However, 500Lmol / L salicylide Acid has a stress effect on pepper. The results showed that exogenous salicylic acid (<500Lmol / L) could significantly increase the disease resistance of TMV-infected chili peppers and was favorable for the adaptation of pepper seedlings to stress.