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目的:研究复发性流产患者蜕膜组织中转化生长因子-β1(TGF-β1)表达及意义。方法:选取2014年3月-2016年3月我院收治的复发性流产患者30例(复发流产组)和人工流产的健康早孕妇女32例(正常妊娠组),采用免疫组织化学法检测TGF-β1的分布和表达情况,Western Blot和RT-PCR分别检测TGF-β1蛋白和mRNA的表达水平。结果:免疫组化结果结果显示:TGF-β1主要表达于蜕膜的腺体细胞和间质细胞,与正常妊娠相比,复发性流产蜕膜组织中的TGF-β1表达明显降低(P<0.05),差异具有统计学意义。荧光定量PCR结果和Western Blot结果与免疫组织化学结果相一致,与正常妊娠相比,复发性流产蜕膜组织中的TGF-β1表达明显降低(P<0.05),差异具有统计学意义。结论:复发性流产蜕膜组织中TGF-β1的表达降低,提示TGF-β1对妊娠的维持发挥重要作用。
Objective: To study the expression of transforming growth factor-β1 (TGF-β1) in decidua of patients with recurrent spontaneous abortion and its significance. Methods: From March 2014 to March 2016, 30 patients with recurrent spontaneous abortion (recurrent spontaneous abortion) and 32 healthy early pregnant women (normal pregnancy group) with induced abortion were enrolled in this study. Immunohistochemistry was used to detect the expression of TGF- β1, and the expression of TGF-β1 protein and mRNA were detected by Western Blot and RT-PCR. Results: The results of immunohistochemistry showed that TGF-β1 was mainly expressed in the decidual glandular cells and interstitial cells, and the expression of TGF-β1 in decidua was significantly lower than that in normal pregnancy (P <0.05 ), The difference was statistically significant. Fluorescent quantitative PCR results and Western Blot results were consistent with immunohistochemical results. Compared with normal pregnancy, the expression of TGF-β1 in decidua of recurrent spontaneous abortion was significantly lower (P <0.05), and the difference was statistically significant. Conclusion: The expression of TGF-β1 is decreased in recurrent spontaneous abortion, suggesting that TGF-β1 plays an important role in the maintenance of pregnancy.