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目的 研究三氧化二砷 (As2 O3 )对人肺腺癌的抑制和诱导凋亡作用及对LRP、MRP基因表达的影响及可能机制。方法 哈尔滨医科大学第一临床医院呼吸内科于 2 0 0 2 - 0 5～ 2 0 0 4 - 0 6选用人肺腺癌A5 4 9细胞株 ,运用体外细胞培养法 ,MTT法 ,流式细胞术检测As2 O3 对人肺腺癌的抑制和诱导凋亡作用 ;用逆转录 -聚合酶链反应(RT -PCR)方法检测LRP、MRPmRNA的表达。结果 As2 O3 对人肺腺癌A5 94细胞具有抑制作用 ,其抑制率呈时间 -剂量依赖关系。不同浓度的As2 O3 均可诱导凋亡。 1 0 μmol/L、2 0 μmol/L的As2 O3 可下调LRP、MRPmRNA的表达。结论 As2 O3 具有抗肿瘤作用 ,主要是通过诱导细胞凋亡实现的 ,其机制与下调LRP、MRPmRNA表达有密切关系。
Objective To investigate the effects of arsenic trioxide (As2 O3) on human lung adenocarcinoma cells and their effects on the expression of LRP and MRP and their possible mechanisms. Methods Human lung adenocarcinoma A549 cell line was selected from Department of Respiratory Medicine, the First Clinical Hospital of Harbin Medical University from 2000-2005 to 2.04-0.6 and cultured in vitro by cell culture method, MTT method, flow cytometry The inhibitory effect of As2O3 on human lung adenocarcinoma and the induction of apoptosis were detected. The expression of LRP and MRP mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Results As2 O3 had an inhibitory effect on human lung adenocarcinoma A594 cells in a time-and dose-dependent manner. Different concentrations of As2 O3 can induce apoptosis. 1 0 μmol / L, 20 μmol / L As2 O3 down-regulated the expression of LRP and MRP mRNA. Conclusion As2 O3 has anti-tumor effect, mainly through the induction of apoptosis, and its mechanism is closely related to the down-regulation of LRP and MRP mRNA expression.