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目的 :探讨缺氧耐受形成中脑内一氧化氮 ( NO)减少的机理。方法 :采用高压液相 Acc Q·Tag柱前衍生法测定缺氧耐受形成中小鼠脑内 L-精氨酸含量 ,双波长分光光度法测定缺氧耐受形成中小鼠脑内一氧化氮合成酶 ( NOS)活性。结果 :L-精氨酸 :1次缺氧组与正常对照组比有升高的趋势 ,但无统计学意义 ( P>0 .0 5) ;2 ,3,4次缺氧组比 1次缺氧组明显下降 ( P<0 .0 5) ;2 ,3,4次缺氧组比正常对照组有下降的趋势 ,但无统计学意义 ( P>0 .0 5) ;1次缺氧组与正常对照组比NOS活性显著升高 ;4次缺氧组与 1次缺氧组比有下降趋势 ,但无统计学意义 ( P>0 .0 5)。结论 :脑内 L-精氨酸含量减少及 NOS活性增加的抑制 ,导致脑内 NO合成减少 ,促进了缺氧耐受性的形成。
Objective: To investigate the mechanism of mid-brain nitric oxide (NO) reduction caused by hypoxia tolerance. Methods: The content of L-arginine in brain of hypodermic mice was measured by Acc Q · Tag pre-column derivatization method and the nitric oxide synthesis in brain of mice was determined by dual-wavelength spectrophotometry Enzyme (NOS) activity. Results: L-arginine: Compared with the normal control group, the level of L-arginine in the first hypoxia group had an increasing trend, but there was no statistical significance (P> 0.05) The hypoxic group decreased significantly (P <0.05); the hypoxic group on the 2nd, 3rd and 4th hypoxic groups showed a decreasing trend compared with the normal control group, but there was no statistical significance (P> 0.05) Compared with the normal control group, the activity of NOS was significantly increased. The ratio of the 4th hypoxia group to the 1st hypoxia group had a decreasing trend, but there was no statistical significance (P> 0.05). Conclusion: The decrease of L-arginine and the increase of NOS activity in the brain lead to the decrease of NO synthesis in the brain and promote the formation of hypoxia tolerance.