论文部分内容阅读
目的:建立脑心络通丸的质量标准。方法:对方中的黄芪、僵蚕、全蝎采用显微鉴别;对桃仁、黄芪、白芍、大黄、牡丹皮和丹参采用TLC鉴别;采用HPLC法测定总阿魏酸的含量,色谱柱:Sapphire C_(18)柱(250 mm×4.6 mm,5μm),流动相:乙腈-0.1%磷酸(12∶88),检测波长:316 nm,柱温:35℃,流速:1.0 ml·min~(-1)。结果:黄芪、僵蚕、全蝎的显微特征明显,易察见;桃仁、黄芪、白芍、大黄、牡丹皮和丹参的TLC色谱图斑点清晰,专属性强;阿魏酸在5.1~81.6μg·ml~(-1)浓度范围内线性关系良好(r=0.999 9),平均回收率为103.30%,RSD为1.96%(n=9)。结论:所建立的标准可用于脑心络通丸的质量控制。
Objective: To establish the quality standard of Naoxintongtong Pills. Methods: Astragalus, silkworm and scorpion were identified by microscopy. The contents of total ferulic acid were determined by HPLC for peach kernel, astragalus membranaceus, white peony root, rhubarb, tree peony bark, and Salvia miltiorrhiza Bunge. The chromatographic column was Sapphire The mobile phase was acetonitrile-0.1% phosphoric acid (12:88), detection wavelength was 316 nm, column temperature was 35 ℃, flow rate was 1.0 ml · min ~ (- 1). Results: The microscopic features of Astragalus, silkworm, scorpion were obvious and easy to see. The TLC chromatogram of peach kernel, Astragalus membranaceus, Radix Paeoniae Alba, rhubarb, moutan and Salvia miltiorrhiza were clear and specific; the ferulic acid was in the range of 5.1 ~ 81.6 The linear relationship was good (r = 0.999 9) with the average recovery of 103.30% and RSD 1.96% (n = 9). Conclusion: The established standards can be used for quality control of Naoxintongtong pill.