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1 材料和方法清洁型 Wistar♂大鼠70只,随机分为正常对照组(10只)、模型对照组(30只)和干扰素治疗组(30只).参照 Montfort 造模方法,应用40%(V/V)CCl_4纯花生油溶液按2mL/kg 体重予干扰素治疗组及模型对照组 Wistar 大鼠ip,2次/wk,共6wk.肝脏经病理检查确定为肝纤维化早期,造模完毕后干扰素治疗组以重组α-IFN sc,10~4 u/d,共3wk;模型对照组以相应体积生理盐水 sc.取两组用药1 wk,2wk,3wk 及正常组大鼠肝脏标本除留作常规 HE 染色外,其余迅速投入液氮保存.根据Caenbank 资料设计间质胶原酶(matrix metalloproteinase-1,MMP-1)及内参对照β-actin 基因序列引物,以总 RNA 提取试剂
1 Materials and Methods Seventy Wistar male rats were randomly divided into normal control group (n = 10), model control group (n = 30) and interferon treatment group (n = 30) (V / V) CCl_4 pure peanut oil solution according to 2mL / kg body weight interferon treatment group and model control group Wistar rats ip, 2 times / wk, a total of 6wk. Liver pathological examination identified as early liver fibrosis, modeling completed Interferon treatment group with recombinant α-IFN sc, 10 ~ 4 u / d, a total of 3wk; model control group with the corresponding volume of normal saline sc. Take two groups of drugs 1 wk, 2wk, 3wk and normal liver samples Leaving for routine HE staining, the other quickly put into liquid nitrogen preservation.According to Caenbank data design matrix metalloproteinase-1 (MMP-1) and internal control reference β-actin gene sequence primers, total RNA extraction reagent