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目的探讨颞叶癫癎大鼠脑内 Sema3A 及其受体 Np1的表达变化在癫癎发病中的作用。方法 SD 大鼠制成颞叶癫癎模型,Neo-Timm 染色证实苔藓纤维出芽,免疫组化和原位杂交技术对致癎后不同时间点内嗅皮质和齿状回的 Sema3A mRNA、Np1 mRNA 和蛋白表达进行分析。结果与对照组比较,颞叶癫癎大鼠海马齿状回内分子层存在苔藓纤维出芽(7 d:0.70±0.42,15 d:1.50±0.52,30 d:2.20±0.41,60 d:2.50±0.51,P<0.05);在匹罗卡品致癎后7 d,实验组内嗅皮质区Sema3A mRNA 的表达(0.3006±0.0675)明显低于对照组(0.4562±0.0457,P<0.01);齿状回内 Np1mRNA(0.2337±0.0358)及蛋白(0.2706±0.0389)的表达亦明显低于对照组(P<0.01)。结论内嗅皮质区 Sema3A mRNA、齿状回内 Np1 mRNA 和蛋白的表达下调,可能参与了苔藓纤维的出芽机制。
Objective To investigate the role of Sema3A and its receptor Np1 in temporal lobe epileptic rat brain in the pathogenesis of epilepsy. Methods The temporal lobe epilepsy model was made in SD rats. The sprouting of moss fibers, the expression of Sema3A mRNA and Np1 mRNA in the olfactory cortex and the dentate gyrus at different time points were detected by Neo-Timm staining, immunohistochemistry and in situ hybridization Protein expression was analyzed. Results Compared with the control group, mossy fiber sprouting occurred in the inner lamina of hippocampal dentate gyrus in temporal lobe epileptic rats (7 d: 0.70 ± 0.42, 15 d: 1.50 ± 0.52, 30 d: 2.20 ± 0.41, 60 d: 2.50 ± 0.51, P <0.05). After 7 days of pilocarpine, the expression of Sema3A mRNA in the entorhinal cortex (0.3006 ± 0.0675) in the experimental group was significantly lower than that in the control group (0.4562 ± 0.0457, P <0.01) The expression of Np1mRNA (0.2337 ± 0.0358) and protein (0.2706 ± 0.0389) were also significantly lower than those of the control group (P <0.01). Conclusions The expression of Sema3A mRNA and Np1 mRNA and protein in the entorhinal cortex is downregulated, which may be involved in the sprouting of mossy fibers.