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为研究硒保护实验动物大脑表面微循环免受自由基损伤红细胞的干扰作用、以及术前服硒对心脏手术病人在体外循环过程中皮肤和肌肉微循环的保护效果，在动物实验中利用荧光标记的图象分析法观察大鼠脑微小动静脉直径、流速和通透性的改变，并在临床观察中用激光多普乐法对服硒组心脏手术病人皮肤和肌肉微循环的变化与对照组进行比较研究。结果表明：尽管硒不能改善已受自由基损伤红细胞的变形性，但硒与受自由基损伤的红细胞同时输入不仅可有效防止这些红细胞引起的大鼠脑微小动、静脉直径的缩小、流速的下降和通透性的增大，同时还可适度提高微动脉的流速；服硒使病人术前皮肤升温后最大微循环灌注量与此时皮肤平均微循环灌注量的比值较对照组显著增加（服硒组增加3．95倍，对照组增加1．74倍，P＜0．05），术中自由基产生水平降低、红细胞膜分子流动性和红细胞变形性无特征性改变，但与对照组的皮肤和肌肉微循环变化趋势相同；服硒组术后24h的皮肤平均微循环灌注量几乎回复到术前水平，肌肉微循环恢复不明显。结论：（1）自由基损伤的红细胞可造成微循环明显恶化，（2）微量元素硒可有效地防止受自由基损伤的红细胞对微循环的影响，（3）术前服硒可提高病人术前皮肤微循环的最大灌注量、防止术中红细胞?
In order to study the interference of selenium on the brain surface microcirculation of the selenium-protected animals from free radical damage and the protective effect of preoperative selenium on the microcirculation of skin and muscle during cardiopulmonary bypass in selenium-treated patients, fluorescent labeling The image analysis method was used to observe the changes of the diameter, velocity and permeability of the cerebral arterioles, and to observe the changes of microcirculation in skin and muscle of patients undergoing cardiac surgery with selenium-enriched selenium by laser Dopamine method in clinical observation. Make a comparative study. The results showed that, while selenium could not improve the deformability of erythrocytes that had been damaged by free radicals, the simultaneous input of selenium and erythrocytes damaged by free radicals could not only effectively prevent the narrowing of venules and the decrease of venous diameter caused by these erythrocytes, but also decrease the flow velocity And permeability increased, but also can moderately increase the velocity of arterioles; serving selenium patients preoperative skin warming after the maximum microcirculation perfusion volume and the average skin microcirculation perfusion ratio was significantly increased compared with the control group Selenium group increased 3.95 times, control group increased 1.74 times, P <0.05), lower levels of free radical production during surgery, the molecular mobility of erythrocyte membrane and erythrocyte deformability no characteristic changes, but with the control group The change trend of microcirculation in skin and muscle were the same. The mean microcirculation perfusion of selenium in group wearing selenium almost returned to preoperative level at 24h, and the recovery of muscle microcirculation was not obvious. Conclusion: (1) Free radical damage of red blood cells can cause significant deterioration of microcirculation, (2) trace element selenium can be effectively prevented by free radical damage of red blood cells on microcirculation, (3) preoperative selenium can improve the patient’s surgery Before the maximum perfusion of skin microcirculation, to prevent intraoperative red blood cells?