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[目的]对单增李斯特菌鉴定方法进行研究。[方法]分别用API检测方法、16SrRNA序列分析法和焦磷酸测序法对17株单核细胞增生李斯特菌进行检测。[结果]3种方法均能准确鉴定单增李斯特菌。[结论]焦磷酸测序方法检测单增李斯特菌特异性强、经济、操作简便、检测快速,为单增李斯特菌的快速、高通量检测提供了一种新的手段。
[Objective] To study the identification of Listeria monocytogenes. [Method] 17 strains of Listeria monocytogenes were detected by API assay, 16S rRNA sequence analysis and pyrosequencing. [Result] All three methods could accurately identify Listeria monocytogenes. [Conclusion] The pyrosequencing method for detection of Listeria monocytogenes is strong, specific, economical, easy to operate and fast to detect, providing a new method for rapid and high throughput detection of Listeria monocytogenes.