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为了研究大鼠孕期染锰后 ,其子代脑组织一氧化氮合酶活性的变化 ,对妊娠大鼠腹腔注射氯化锰 ,剂量为 0、7.5、15、30 mg/ kg,然后对其子代 30日龄仔鼠脑组织用组织化学法检测一氧化氮合酶活性 ,并用图象分析仪定量分析。结果表明 :实验各组仔鼠脑组织一氧化氮合酶阳性神经元的截面积、平均吸光度、积分吸光度均明显高于对照组 ( P<0 .0 1)。大鼠孕期染锰 ,使其子代脑组织一氧化氮合酶活性增强 ,促使一氧化氮合成增多 ,从而引起中枢神经受损
In order to study the changes of nitric oxide synthase activity in the brain of the offsprings of pregnant rats exposed to manganese, the pregnant rats were injected intraperitoneally with manganese chloride at doses of 0, 7.5, 15 and 30 mg / kg, The activity of nitric oxide synthase was detected by histochemistry in the brains of 30-day-old offspring rats and quantified by image analyzer. The results showed that the cross-sectional area, average absorbance and integral absorbance of nitric oxide synthase positive neurons in the brain of each group were significantly higher than those of the control group (P <0.01). Manganese in pregnancy, so that the offspring of brain tissue nitric oxide synthase activity increased, leading to an increase in nitric oxide synthesis, which led to central nervous system damage