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本文在细胞水平上研究了 N-乙酰 L-半胱氨酸 ( NAC)及还原型谷胱甘肽 ( GSH)对抗硫芥致细胞坏死及凋亡的效应 .结果表明 :NAC和 GSH均可部分地对抗硫芥 1 0 0 0μmol· L-1引起的细胞坏死 ,可明显提高细胞的存活率 ,二者的有效浓度为0 .1 - 1 0 mmol· L-1. NAC( 5mmol· L-1)和 GSH( 5mmol· L-1)还均可对抗硫芥 ( 1 0 0 μmol·L-1)引起的细胞凋亡 ,使 DNA的降解片段明显减少 .流式细胞术检测表明 ,硫芥 1 0 0 μmol· L-1作用 1 2 h的 Hela细胞凋亡百分率为 37.2 % ,而 NAC和 GSH保护组的细胞凋亡百分率分别为 5.2 % ,8.2 % .谷氨酰胺也有对抗硫芥细胞毒的作用 ,但保护效果不及 NAC和 GSH.
The effects of N-acetyl-L-cysteine (NAC) and reduced glutathione (GSH) on cell-induced necrosis and apoptosis in Sulfur mustard were studied at the cellular level.The results showed that both NAC and GSH could partially In contrast to the cell necrosis caused by sulfurous mustard 100 micromol · L-1, the cell viability was significantly increased, the effective concentration of both was 0.1-1.0 mmol · L-1. NAC (5 mmol · L -1 ) And GSH (5 mmol · L-1) could also counteract the apoptosis induced by sulfur mustard (100 μmol·L-1), and significantly reduce the degradation of DNA.Flow cytometry showed that sulfur mustard 1 The percentage of apoptosis of Hela cells treated with 0 μmol·L-1 for 1 2 h was 37.2%, while the percentages of apoptotic cells in NAC and GSH-treated groups were 5.2% and 8.2%, respectively. Glutamine also inhibited the cytotoxicity against sulfur mustard Effect, but less effective than NAC and GSH.