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目的:观察水通道蛋白1(Aquaporin 1,AQP1)、水通道蛋白2(Aquaporin 2,AQP2)、水通道蛋白3(Aquaporin 3,AQP3)在膀胱尿路上皮癌组织及正常膀胱组织中的表达与分布,探讨其意义。方法:取膀胱尿路上皮癌组织25例及正常膀胱组织25例,应用免疫组织化学方法检测AQP1、AQP2和AQP3在膀胱尿路上皮癌和正常膀胱组织中的表达及分布。结果:AQP1散在表达于正常膀胱组织中微血管和小动脉的内皮细胞的细胞膜及细胞质,在膀胱尿路上皮癌组织中AQP1大量表达于肿瘤的血管内皮细胞的细胞膜及细胞质;膀胱尿路上皮癌中AQP1表达水平较正常膀胱组织明显增高,差异有统计学意义(P<0.05);AQP2主要表达于膀胱尿路上皮癌组织及正常膀胱组织的细胞间质中,两者表达强度基本相同,差异无统计学意义(P>0.05);AQP3主要表达在膀胱尿路上皮癌组织及正常膀胱组织的黏膜上皮细胞的胞质及胞膜,在癌组织中的表达强度明显增高,差异有统计学意义(P<0.05)。结论:AQP1、AQP3在膀胱尿路上皮癌组织中表达明显增高。提示AQP1、AQP3可能与膀胱尿路上皮癌的发生和发展密切相关,其机制需要进一步深入研究。
Objective: To investigate the expression of AQP1, AQP2 and AQP3 in bladder urothelial carcinoma and normal bladder tissues Distribution, explore its significance. Methods: Twenty-five cases of bladder urothelial carcinoma and 25 cases of normal bladder tissue were obtained. The expression and distribution of AQP1, AQP2 and AQP3 in bladder urothelial carcinoma and normal bladder tissues were detected by immunohistochemistry. Results: AQP1 was mainly expressed in the plasma membrane and cytoplasm of endothelial cells in the microvessels and arterioles in normal bladder tissues. AQP1 was abundantly expressed in the plasma membrane and cytoplasm of vascular endothelial cells in bladder urothelial carcinoma tissues. In bladder urothelial carcinoma The expression of AQP1 in normal bladder tissue was significantly higher than that in normal bladder tissue (P <0.05). AQP2 was mainly expressed in the cytoplasm of bladder urothelial carcinoma and normal bladder tissue, and the expression intensity of AQP1 was almost the same (P> 0.05). AQP3 was mainly expressed in the cytoplasm and membrane of mucosal epithelial cells in bladder urothelial carcinoma tissues and normal bladder tissues, and the expression intensity of AQP3 in cancer tissues was significantly increased (P> 0.05) P <0.05). Conclusion: The expression of AQP1 and AQP3 in bladder urothelial carcinoma was significantly increased. These results suggest that AQP1 and AQP3 may be closely related to the occurrence and development of bladder urothelial carcinoma. The mechanism needs further study.