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目的建立全血中氯美扎酮的全自动固相萃取/气相色谱质谱联用检测方法。方法采用waters Oasis HLB Cartridge固相萃取柱,甲醇活化,去离子水淋洗,二氯甲烷洗脱,GC/MS定性、定量检验,并对固相萃取条件和检测条件进行评价。结果全自动固相萃取全血中氯美扎酮平均萃取回收率达89%以上,最低检出限5ng/mL,专属性,精密度达标。结论该方法操作简便快速,选择性好,提取物杂质少,定性定量准确。可用于实际案件的检验。
OBJECTIVE To establish a full-automatic solid phase extraction / GC-MS detection method of Clomipramine in whole blood. Methods Waters Oasis HLB cartridge solid phase extraction column, methanol activation, deionized water rinse, dichloromethane elution, GC / MS qualitative and quantitative test, and solid-phase extraction conditions and testing conditions were evaluated. Results The average recovery of chlormethazar in whole blood was over 89% and the lowest detection limit was 5 ng / mL. The specificity and precision reached the standard. Conclusion The method is simple and rapid in operation with good selectivity, less impurities and qualitative and quantitative analysis. Can be used to test the actual case.