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目的研究长期饮酒对急性中毒大鼠体液中亚甲基二氧甲基苯丙胺(MDMA)死后再分布的影响。方法 SD雄性大鼠360只,随机均分为A、B、C、D 4组;A、B组以白酒,C、D组以双蒸水为饮用液体,4周后各组按150mg/kg MDMA剂量灌胃,处死后分置于25℃、4℃条件下;以VARIAN CP-3800气相色谱仪分别检测处死时血乙醇含量和0～10d内体液样品中MDMA浓度。结果 0～10d不同条件下,大鼠血液、玻璃体液及尿液中MDMA的PMR浓度变化趋势均为先升高、后降低;各时间点A、B组和C、D组大鼠各体液样本MDMA浓度较0h均有显著性差异(P<0.05),各时间点A与C组、B与D组之间体液样本MDMA浓度有显著性差异(P<0.05);A与B组、C与D组之间体液样本MDMA浓度有显著性差异(P<0.05)。结论长期饮用乙醇会降低MDMA在体液样品中的再分布,其影响程度高低依次为血液、尿液及玻璃体液;低温也可减少体液中MDMA的再分布。
Objective To study the effect of long-term alcohol consumption on the post-mortem redistribution of MDMA in body fluids of acute poisoning rats. Methods 360 male SD rats were randomly divided into A, B, C and D 4 groups. A and B groups were drunk with distilled water as drinking liquid in group C and D, and after 150 mg / kg MDMA was intragastrically administered. After sacrificed, the cells were separated and placed at 25 ℃ and 4 ℃. Blood ethanol concentration and MDMA concentration in body fluid samples were detected by VARIAN CP-3800 gas chromatograph. Results Under different conditions from 0 to 10 days, the change trends of PMR concentration in blood, vitreous fluid and urine of MDMA rats first increased and then decreased. The body fluid samples of A, B and C, D groups at each time point (P <0.05). There was significant difference of MDMA concentration in body fluid sample between A and C group, B group and D group at each time point (P <0.05) There was a significant difference in MDMA concentration between the two groups (P <0.05). Conclusion Long-term consumption of ethanol will decrease the redistribution of MDMA in the body fluid samples, with the order of blood, urine and vitreous humor. The hypothermia may also reduce the redistribution of MDMA in body fluids.