为了探明草鱼出血病病毒的血凝作用及其影响因素 ,应用血凝试验对此进行了测定 ,结果表明 :病毒只对鸡红细胞引起高滴度凝集 ,这种凝集能被特异性抗血清所抑制 ;血凝的适宜 pH为 5 .5～ 7.0 ,2 %的氯化钠为较适宜的稀释液 ,血凝活性以在 37℃温度条件下最好 ;出血病病鱼肠道的凝集价最高 ;醛化的鸡红细胞在 4℃下保存一年多仍具有新鲜红细胞表面受体活性 ,从而使草鱼出血病的HA和HI试验更具有稳定性和广泛的实用性 ,建立了一种快速诊断草鱼出血病的方法 In order to determine the hemagglutination of grass carp hemorrhagic disease virus and its influencing factors, this was determined by hemagglutination test. The results showed that the virus only caused high titer agglutination of chicken erythrocytes. The agglutination could be blocked by specific antisera The optimum pH value of blood coagulation was 5.5-7.0, 2% sodium chloride was the most suitable diluent, and the hemagglutination activity was the best at 37 ℃. The intestinal agglutination price of hemorrhagic disease fish was the highest ; Aldehyde-based chicken red blood cells stored at 4 ° C for more than a year still have a fresh red blood cell surface receptor activity, so that grass carp hemorrhagic HA and HI test more stability and a wide range of practicality, the establishment of a rapid diagnosis of grass carp Hemorrhagic disease method