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目的:探究大黄治疗脓毒症大鼠的分子机制。方法:100只Wistar大鼠随机分为假手术组,模型组,大黄高、中、低剂量组(150,100,50 mg·kg~(-1)),除假手术组只暴露盲肠,不进行结扎与穿孔,其余各组采用盲肠结扎法制作大鼠脓毒症模型,记录各组大鼠死亡情况,采用细菌16S rRNA实时荧光定量聚合酶链式反应(q PCR)检测大鼠结肠及肠系膜淋巴结、血液中金黄色葡萄球菌及大肠埃希菌数量,采用q PCR和蛋白质免疫印迹(Western blot)法检测大鼠结肠紧密连接蛋白紧密连接相关蛋白-1(ZO-1)和闭锁蛋白(Occludin)水平。结果:与假手术组比较,脓毒症模型组大鼠病死率最高,不同标本的金黄色葡萄球菌及大肠埃希菌检出率和数量明显升高,ZO-1和Occludin mRNA及蛋白表达明显降低(P<0.01);与模型组比较,大黄高、中剂量组大鼠病死率降低,大鼠不同标本的上述两细菌检出率和数量有所减低,细菌移位情况减少,大鼠结肠的ZO-1和Occludin mRNA及蛋白表达明显升高(P<0.05,P<0.01)。结论:大黄可调控结肠紧密连接蛋白表达保护肠黏膜屏障,从而抑制脓毒症大鼠细菌移位治疗脓毒症。
Objective: To explore the molecular mechanism of rhubarb in septic rats. Methods: 100 Wistar rats were randomly divided into sham operation group, model group, rhubarb high, medium and low dose groups (150, 100 and 50 mg · kg -1). Except for the sham group, only the cecum was exposed without ligation The mice in each group were sacrificed by cecal ligation to make sepsis model. The deaths of rats in each group were recorded. The colon and mesenteric lymph nodes of rats were detected by 16S rRNA real-time polymerase chain reaction (qPCR) The quantity of Staphylococcus aureus and Escherichia coli in the blood were detected by q PCR and Western blotting to detect the expression of ZO-1 and Occludin . Results: Compared with the sham-operation group, the mortality of sepsis model group was the highest, the detection rate and quantity of Staphylococcus aureus and Escherichia coli in different samples were significantly increased, and the expression of ZO-1 and Occludin mRNA and protein were obvious (P <0.01) .Compared with the model group, the mortality of rats in high and middle dose rhubarb group decreased, the detection rate and quantity of the two bacteria in different samples of rats decreased, the bacterial translocation decreased, The mRNA and protein expressions of ZO-1 and Occludin were significantly increased (P <0.05, P <0.01). Conclusion: Rhubarb can regulate colon tight junction protein expression and protect the intestinal mucosal barrier, thus inhibiting bacterial translocation in septic rats for sepsis.