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以转cry1Ac/sck双价抗虫基因工程稻MSA4、KF6-304、21S/MSB与II-32A/MSB大米为材料,进行了小鼠急性毒性试验、精子畸形试验与骨髓细胞微核试验研究,检测转基因稻大米对小鼠表观体症行为、脏器系数、血常规、精子畸形与微核率等的影响,以进一步评价转基因工程稻大米的食品安全性.小鼠急性毒性试验结果显示:转基因工程稻MSA4、KF6-304、21S/MSB与I-I-32A/MSB大米各剂量组灌喂小鼠,小鼠的行为、姿势、呼吸、毛色均正常;解剖观察其肝、脾、肾、心脏、胃、肠、生殖腺等主要脏器的外形、颜色、大小比例等无异常;血常规分析也显示受试鼠与对照材料组小鼠没有显著差异.小鼠精子畸形试验与骨髓细胞微核试验结果均显示转基因工程稻没有明显诱发小鼠精子畸变与骨髓细胞产生微核.其结果是:转基因工程稻MSA4、KF6-304、21S/MSB与II-32A/MSB大米未引起小鼠明显的急性毒性反应,对小鼠无明显的致畸与致突变作用.
The acute toxicity test, sperm abnormality test and myeloid micronucleus test were carried out in transgenic rice plants cry1Ac / sck with MSA4, KF6-304, 21S / MSB and II-32A / MSB. To detect the effect of transgenic rice on the apparent symptom, organ coefficient, blood routine, sperm deformity and micronucleus rate in transgenic mice to further evaluate the food safety of the transgenic rice.Acute toxicity test results in mice showed that: The mice in each dose group of genetically engineered rice MSA4, KF6-304, 21S / MSB and II-32A / MSB rice were normal in their behavior, posture, respiration and coat color. The liver, spleen, kidney and heart were dissected , Stomach, intestine, gonads and other major organs of the shape, color, size and other proportions without abnormalities; blood tests also showed no significant difference between the experimental and control groups of mice.Mice sperm deformity test and bone marrow micronucleus test The results showed that transgenic rice did not induce mouse sperm aberration and produce micronuclei in bone marrow cells.The results showed that transgenic rice lines MSA4, KF6-304, 21S / MSB and II-32A / MSB did not cause obvious acute Toxicity Should, no obvious teratogenic and mutagenic effects on mice.