目的:观察人牙周膜细胞静压力下细胞内蛋白质表达的变化,为进一步探讨静压力与人牙周膜细胞代谢之间的关系奠定基础。方法:采用固相pH梯度/SDS-PAGE双向电泳和质谱分析技术,对人牙周膜细胞静压力干预组和未加力组细胞胞内总蛋白质进行分析,应用Image Master 2D Platinum Software 5.0分析软件,鉴定2组细胞的双向电泳图谱,差异表达的蛋白质点为30个,选择其中5个新出现的差异点进行质谱分析和数据库检索。结果:第3和第5个蛋白质点得到了鉴定,分别为早老蛋白(presenilin 2)和儿茶酚胺-O-甲基转移酶(catechol O-methyltransferase),2种蛋白质在加力组中新表达。结论:早老蛋白与Notch蛋白具有相关性,而Notch通路可能是参与牙周膜细胞生物力学改建的一条信号传导途径;儿茶酚胺-O-甲基转移酶与降钙素基因相关肽(calcitonin gene-related peptide,CGRP)有关,而CGRP在骨代谢中发挥重要作用。 OBJECTIVE: To observe the changes of intracellular protein expression in human periodontal ligament cells under static pressure, and lay a foundation for further study on the relationship between static pressure and the metabolism of human periodontal ligament cells. Methods: The intracellular total proteins of human periodontal ligament cells in static pressure intervention group and non-stress group were analyzed by solid-phase pH gradient / SDS-PAGE two-dimensional electrophoresis and mass spectrometry. Image Master 2D Platinum Software 5.0 software Two groups of cells were identified by two-dimensional gel electrophoresis. The number of differentially expressed protein spots was 30, and five new spots were selected for mass spectrometry analysis and database search. Results: The third and fifth protein spots were identified as presenilin 2 and catechol O-methyltransferase, respectively. The two proteins were newly expressed in GABA group. CONCLUSION: Presenilin is associated with Notch protein, and Notch pathway may be involved in the biomechanical alteration of periodontal ligament cells. Catecholamine-O-methyltransferase and calcitonin gene-related peptide, CGRP), while CGRP plays an important role in bone metabolism.