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利用SRAP分子标记研究了6个切花菊品种及其2×4不完全双列杂交(NCⅡ)的38个F1代单株的遗传关系。结果表明,17对SRAP引物组合共获得229条带,其中多态性条带127条,平均每个引物获得7.5个多态性条带,多态性比率为56.0%,说明切花菊亲本品种及其杂交后代的分子多样性适中。6个亲本品种之间的Nei’s遗传距离介于0.11~0.25之间,平均为0.19,说明亲本品种之间的亲缘关系较近。亲本和杂交后代的遗传相似系数分别介于0.42~0.72和0.40~0.85之间,杂交后代遗传相似系数的中位数(0.61)高于亲本品种(0.55),说明杂交产生了一些变异株系,但是总的遗传基础有变窄或同质化趋势。基于遗传相似系数,UPGMA聚类将亲本和杂交后代划分为两大类,聚类结果与母本和杂交组合类型相符,说明SRAP分子标记可有效用于鉴定菊花不同杂交组合后代。
The SRAP molecular markers were used to study the genetic relationships among 38 F1 plants of 6 cut chrysanthemum cultivars and 2 × 4 incomplete diallel crosses (NC Ⅱ). The results showed that there were 229 bands with 17 pairs of SRAP primers, of which 127 bands were polymorphic, and 7.5 bands per primer were obtained. The polymorphism rate was 56.0% Its hybrid progeny have moderate molecular diversity. The Nei’s genetic distances among the six parents ranged from 0.11 to 0.25, with an average of 0.19, indicating that the genetic relationship between the parental varieties is relatively close. The genetic similarity coefficients between parents and hybrids ranged from 0.42 to 0.72 and from 0.40 to 0.85, respectively. The median of genetic similarity coefficient (0.61) in hybrids was higher than that in parental lines (0.55) However, the general genetic basis is narrowed or homogenized. Based on the genetic similarity coefficient, UPGMA clustering divides parents and crossing progenies into two groups, and the clustering results are consistent with the maternal and hybrid combinations, indicating that SRAP markers can be effectively used to identify chrysanthemum cross combinations of different cross combinations.