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目的 为观察沙门氏菌疫苗株诱导雌鼠免疫性避孕效应。方法 应用表达鼠精子抗原cyritestin的两种重组沙门氏菌疫苗株X46 32 (pCFL)、X45 5 0 (pCFL)口服免疫雌鼠 ,通过ELISA方法检测其激发雌鼠产生抗重组Cyritestin蛋白和抗菌体LPS(脂多糖 )的血清IgG抗体和阴道分泌物IgA抗体滴度变化。 结果 发现 :两种重组疫苗株不但可激发雌鼠产生高水平血清抗重组Cyritestin蛋白和抗菌体LPSIgG抗体 ,而且在阴道分泌物中可出现相应IgA抗体。同时 ,口服免疫后雌鼠生育能力明显降低 ,两实验组雌鼠妊娠率降低 30 %～ 40 % ,一胎产仔数量平均降低 6 6 .96 %～ 70 .48%。结论 表达精子Cyritestin蛋白的重组沙门氏菌疫苗株可诱导有效的免疫性避孕效应 ,值得深入研究。
The purpose is to observe the immune contraceptive effect induced by Salmonella vaccine in female mice. Methods Two recombinant Salmonella vaccine strains X46 32 (pCFL) and X45 5 0 (pCFL) were immunized with recombinant cyritestin. Female mice were immunized with anti-recombinant Cyritestin and anti-bacterial lipopolysaccharide (LPS) by ELISA Polysaccharide) serum IgG antibody and vaginal secretions IgA antibody titer changes. The results showed that both recombinant vaccines not only induced high levels of serum anti-recombinant Cyritestin protein and anti-LPSIgG antibody in female rats, but also showed corresponding IgA antibodies in vaginal secretions. At the same time, the fertility of female rats decreased significantly after oral immunization. The pregnancy rates of the two experimental groups decreased by 30% -40% and the average number of litter size decreased by 6.66% -70.48%. Conclusions Recombinant Salmonella vaccine strains expressing sperm Cyritestin protein can induce effective immune contraceptive effect, which deserves further study.