论文部分内容阅读
【目的】对一株从连云港海域海藻样品中获得的拮抗细菌Y3F进行鉴定并且研究菌株Y3F对黄瓜土传枯萎病害的控制效果。【方法】对Y3F进行形态、生理生化特征和16S r RNA基因序列分析,采用平板对峙法测定菌株Y3F的活菌液和无菌滤液的抑菌活性,利用盆栽试验测定Y3F对黄瓜枯萎病害的防治效果。【结果】初步鉴定该菌属于蜡样芽孢杆菌(Bacillus cereus),用2216E培养基振荡培养24 h的Y3F的无菌滤液对黄瓜尖孢镰刀菌有较强的抑菌活性,表明菌株能分泌抑制病原菌生长的活性物质。盆栽试验表明,种植30 d后,浸种和灌根同时处理(JG)的防治效果达到50.46%,对黄瓜枯萎病害有明显的控制效果,显著提高黄瓜植株生物量,显著降低黄瓜根际土的真菌和尖孢镰刀菌数量,增加根际的细菌和放线菌数量。【结论】菌株Y3F能有效防治黄瓜枯萎病害,改善根际微生物结构,具有进一步开发应用的前景。
【Objective】 The objective of this study was to identify an antagonistic bacterium Y3F obtained from seaweed samples collected from Lianyungang sea area and study the control effect of strain Y3F on cucumber soil wilt disease. 【Method】 The morphological, physiological and biochemical characteristics of Y3F and sequence analysis of 16S rRNA gene were analyzed. The antibacterial activity of the strain Y3F in live and sterile filtrate was determined by plate confrontation method. The pot experiment was conducted to test the control of cucumber wilt disease effect. 【Result】 The results showed that the strain belonged to Bacillus cereus. The sterile filtrate of Y3F cultured in 2216E medium for 24 h had strong antibacterial activity against Fusarium oxysporum, indicating that the strain could inhibit secretion Pathogen growth of active substances. The results of pot experiment showed that the control effect of soaking and flooding simultaneously (JG) reached 50.46% after 30 d of planting, which had obvious control effect on the cucumber wilt disease, significantly increased cucumber plant biomass and significantly reduced the fungi in cucumber rhizosphere soil And Fusarium oxysporum to increase the number of rhizosphere bacteria and actinomycetes. 【Conclusion】 The strain Y3F can effectively prevent the cucumber wilt disease and improve the rhizosphere microbial structure, which has the prospect of further development and application.