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小麦条锈病是世界范围内小麦上最重要的流行性病害之一,可造成严重的产量损失。陇南地区是我国小麦条锈菌主要越夏易变区和新小种发源地,了解该地区不同海拔高度区域内条锈菌遗传多样性有重要意义。本研究采用TP-M13-SSR荧光标记技术对11个种群330个小麦条锈菌分离株基因组DNA进行了SSR标记分析。不同海拔区域的条锈菌遗传多样性有明显的差异,高山区的遗传多样性比较丰富,半山区次之,川道区相对比较低。不同生态区域内,小麦条锈菌群体遗传分化程度不同,高山区和半山区遗传分化程度大,基因流小,川道区群体遗传分化程度比较小,基因流大。来自不同海拔区域的菌系具有相同的基因型,这一结果从分子水平证明了在陇南地区小麦条锈菌在山区与川地之间存在广泛的菌源交流,可就地完成周年循环。
Wheat stripe rust is one of the most important epidemic diseases in wheat worldwide and can cause serious yield losses. Longnan area is the main area of wheat stripe rust overhangs and new races in China. It is important to know the genetic diversity of stripe rust in different altitudes in this area. In this study, genomic DNA of 330 stripe rust isolates from 11 populations was analyzed by SSR markers using TP-M13-SSR fluorescent labeling. The genetic diversity of stripe rust in different altitudes is obviously different. The genetic diversity of high-mountain area is rich, followed by Mid-mountain area and relatively low in Chuandao area. In different ecological regions, the degree of genetic differentiation of wheat stripe rust population was different. The genetic differentiation degree in mountainous and semi-mountainous areas was large and the gene flow was small. The degree of genetic differentiation was relatively small and the gene flow was large in the population of the Chuandao district. The bacterial strains from different altitudes have the same genotype. This result proves at the molecular level that there is a wide range of bacterial source communication between the wheat stripe rust in the Longnan region and the Sichuan region, and the annual cycle can be completed on the spot.