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目的 探讨视神经部分损伤后视网膜、视神经基因表达谱的变化。 方法 60只SD大鼠随机分成4组,反向镊夹持大鼠右眼视神经6s ,分别于伤后3、7、14、2 1d取手术眼及对侧假手术眼(仅暴露但不夹持视神经)的视神经和视网膜,利用高密度基因芯片技术检测基因表达谱的变化。 结果 视神经损伤后有大量基因表达发生改变,伤后3、7、14、2 1d的阳性基因表达率分别为2 .3 5 %、6.48%、3 .82 %和4 0 9% ,总阳性表达率为11.77% ,阳性表达基因的功能包括细胞生长和存活、细胞骨架、细胞外基质和细胞粘附、自由基和氧化损伤、能量和代谢、炎症、神经传递和离子转运、细胞信号转导、结构蛋白、转录和翻译等。修复基因的表达上调或下调是基因表达变化的主体,破坏基因的变化占的比例较小,其中伤后7d表达上调的修复基因占表达改变的基因的13 .98% ,表达下调的修复基因占2 4.73 % ,伤后14d表达下调的修复基因占17.2 0 %。 结论 视神经损伤后有大量的基因表达发生改变,全面了解视神经损伤后基因表达的变化对于深入研究损伤后反应特别是再生反应的机制具有重要意义。
Objective To investigate the changes of gene expression profile of retina and optic nerve after partial optic nerve injury. Methods Sixty Sprague-Dawley rats were randomly divided into four groups. The contralateral tweezers were used to hold the right optic nerve of the rats for 6 seconds. The injured eyes were exposed at 3, 7, 14 and 21 days respectively Optic nerve and the retina holding the optic nerve), and detecting the change of gene expression profile by using high-density gene chip technology. Results A large number of gene expressions changed after optic nerve injury. The positive expression rates of the positive genes at 3, 7, 14 and 21 days after injury were 2.5%, 6.48%, 3.82% and 409.0%, respectively. (11.77%). Positively expressed genes include cell growth and survival, cytoskeleton, extracellular matrix and cell adhesion, free radical and oxidative damage, energy and metabolism, inflammation, neurotransmission and ion transport, cell signaling, Structural proteins, transcription and translation. The up-regulated or down-regulated expression of the repair gene was the main body of the change of gene expression, and the proportion of the disrupted gene accounted for a small proportion, of which 13.98% of the gene whose expression was up-regulated was expressed at 7d after injury, 2 4.73%. The repair genes that were down-regulated 14 days after injury accounted for 17.2%. Conclusion A large number of gene expression changes after optic nerve injury. To understand the changes of gene expression after optic nerve injury is of great significance for further study on the mechanism of post-injury reaction, especially regeneration.