论文部分内容阅读
目的 :探讨大剂量、长时间补充肌酸对大鼠自身肌酸生物合成过程和肌酸代谢的影响。方法 :测定不同剂量补充肌酸 4周后大鼠肾脏的精氨酸 -甘氨酸脒基转移酶 (AGAT)活性和酶促反应产物胍乙酸含量、骨骼肌肌酸含量、血清肌酸激酶活性和肌酐水平。结果 :补充 4周不同剂量的肌酸后 ,大鼠体重有增加的趋势 ,同时腓肠肌湿重显著增加 (P <0 0 5 ) ,但 6 0g/kg组大鼠的体重和腓肠肌湿重低于其它剂量组。肾脏AGAT活性和胍乙酸含量有不同程度的降低 (P <0 0 1) ,补充剂量越大 ,降低程度越明显。结论 :大剂量、长时间补充肌酸可显著影响内源性肌酸的合成。超大剂量补充肌酸还会造成腹泻和骨骼肌量丢失。研究结果提示 ,补充肌酸应适量适时 ,不可长时间大剂量或超大剂量使用。
Objective: To investigate the effects of high dose and prolonged creatine supplementation on creatine biosynthesis and creatine metabolism in rats. Methods: The arginine-glycine amidotransferase (AGAT) activity and the content of guanidine acetic acid, creatine content in skeletal muscle, serum creatine kinase and creatinine in kidney of rats were measured after 4 weeks of different doses of creatine supplementation Level. Results: After 4 weeks of different doses of creatine supplementation, the body weight of rats increased while the wet weight of gastrocnemius muscle increased significantly (P <0.05). However, the body weight and gastrocnemius wet weight of rats in 60 g / kg group were lower than Other dose groups. AGAT activity in kidney and guanidinoic acid content decreased to some extent (P <0.01). The larger the supplementary dose, the more obvious the degree of decrease. Conclusion: High-dose, prolonged creatine supplementation can significantly affect the synthesis of endogenous creatine. Excessive supplementation of creatine can also cause diarrhea and skeletal muscle loss. The results suggest that adequate supplementation of creatine should be timely, not for a long time large dose or large dose.