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通过FT-IR(傅里叶变换红外光谱)和Raman(共焦激光拉曼光谱)分析黄芩苷包合物,对拉曼光谱法作为一种新的验证包合物形成方法的可行性进行验证。采用饱和水溶液法制备黄芩苷包合物,共焦拉曼光谱仪和傅里叶转换红外光谱仪分别测定并获得β-环糊精、黄芩苷、黄芩苷与β-环糊精物理混合物及黄芩苷包合物四种固体粉末的拉曼图谱和红外图谱并将其进行对照分析,这两种方法互相印证,互相补充,可增强鉴定的准确性、可靠性,共同证明了黄芩苷通过氢键作用嵌入β-环糊精的疏水空腔形成包合物,从而增加了它的稳定性。拉曼光谱法具有快速、准确、简便、无损测量等显著优势,可作为验证药物包合物的形成重要手段。
The baicalin inclusion complex was analyzed by FT-IR (Fourier Transform Infrared Spectroscopy) and Raman (Confocal Laser Raman Spectroscopy) to verify the feasibility of Raman spectroscopy as a new method of verifying inclusion complex formation . The baicalin inclusion complex, the confocal Raman spectrometer and the Fourier transform infrared spectrometer were respectively used to prepare the baicalin inclusion complex, baicalin, baicalin and β-cyclodextrin physical mixture and baicalin package Raman and IR spectra of the four solid powders were compared and analyzed. The two methods proved each other and complement each other, which enhanced the accuracy and reliability of the identification and proved that baicalin was embedded by hydrogen bonding The hydrophobic cavity of β-cyclodextrin forms an inclusion complex, increasing its stability. Raman spectroscopy has the obvious advantages of rapid, accurate, simple and nondestructive measurement, which can be used as an important method to verify the formation of drug inclusion.