目的建立高效液相色谱法测定三黄乳膏中黄芩苷含量的方法。方法采用Dikma C18(5μm,250 mm×4.6 mm)色谱柱;流动相为甲醇-水-磷酸(47∶53∶0.2);检测波长为277 nm。结果黄芩苷在0.40~2.40μg/ml浓度范围内与峰面积呈良好的线性关系(r=0.9998 n=6),精密度高,RSD=0.66%,平均回收率98.34%。结论该方法简便,结果准确,重现性好,可作为三黄乳膏的质量控制方法。 Objective To establish a HPLC method for the determination of baicalin in Sanhuang cream. Methods Dikma C18 (5μm, 250 mm×4.6 mm) column was used. The mobile phase was methanol-water-phosphoric acid (47∶53∶0.2). The detection wavelength was 277 nm. Results There was a good linear relationship between peak area and baicalin in the concentration range of 0.40-2.40μg/ml (r=0.9998 n=6). The precision was high, RSD was 0.66%, and the average recovery rate was 98.34%. Conclusion This method is simple, accurate and reproducible. It can be used as a quality control method for Sanhuang cream.