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目的观察瞬时转染信号传导与转录激活因子3(STAT3)小干扰RNA(siRNA)后对人胃癌细胞SGC-7901侵袭力的影响,探讨干扰STAT3后降低胃癌细胞侵袭力的机制。方法以人胃癌细胞系SGC-7901为研究对象,培养瞬时转染特异性siRNA的SGC-7901细胞系,通过RT-PCR、免疫细胞化学、Transwell体外侵袭实验等检测该siRNA对SGC-7901细胞STAT3及MMP-9基因表达和对细胞侵袭转移能力的影响。结果STAT3 siRNA转染SGC-7901细胞48 h后,STAT3 mRNA水平下降了73.04%,MMP-9 mRNA水平下降了57.2%;Transwell体外侵袭实验显示,STAT3 siRNA组能显著抑制SGC-7901的侵袭力(P<0.01)。结论应用siRNA技术能有效抑制SGC-7901STAT3基因的表达,进而可能通过抑制MMP-9基因的表达,降低细胞的侵袭力,为以STAT3为靶向的胃癌基因治疗提供了新的思路和方法。
Objective To investigate the effect of transiently transfected signal transducer and activator of transcription 3 (STAT3) siRNA on invasiveness of human gastric cancer cell line SGC-7901, and to explore the possible mechanism of reducing invasiveness of human gastric cancer cell line STAT3. Methods Human gastric cancer cell line SGC-7901 was cultured in SGC-7901 cells. Transient transfection of SGC-7901 cells with specific siRNA was used to detect the effect of siRNA on STAT3 And MMP-9 gene expression and invasion and metastasis of cells. Results The STAT3 mRNA level was down-regulated by 73.04% and the expression of MMP-9 mRNA by 57.2% after transfection with STAT3 siRNA for 48 h. Transwell invasion assay showed that STAT3 siRNA significantly inhibited the invasiveness of SGC-7901 cells P <0.01). Conclusion The siRNA technology can effectively inhibit the expression of SGC-7901STAT3 gene, which may provide new ideas and methods for the gene therapy of gastric cancer targeting STAT3 by inhibiting the expression of MMP-9 gene and reducing the invasion of cells.