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目的研究青蒿琥酯对佐剂性关节炎大鼠的抗炎免疫机制。方法建立佐剂性关节炎大鼠模型,随机分为空白组、AA模型组、甲氨蝶呤阳性药物对照组和青蒿琥酯大、中、小剂量组(20.0,10.0,2.5 mg.kg.d-1),并灌胃给药。ELISA法检测各组对单核细胞趋化因子(MCP-1)、调节活化T细胞表达和分泌因子(RANTES)和肿瘤坏死因子(TNF-α)的影响。结果青蒿琥酯大、中、小剂量组能显著降低血清TNF-α,与模型组比较差异有统计学意义(P<0.05);青蒿琥酯小剂量组与其大、中剂量组或者与甲氨蝶呤组比较差异有统计学意义(P<0.05);青蒿琥酯各剂量组均能显著降低血清中MCP-l和RANTES的表达,与模型组比较差异有统计学意义(P<0.05);而青蒿琥酯小剂量组与大、中剂量组或者与甲氨蝶呤组比较差异有统计学意义(P<0.05)。结论青蒿琥酯抗炎和免疫调节作用可能是与抑制炎症因子相关。
Objective To study the anti-inflammatory immune mechanism of artesunate on adjuvant arthritis rats. Methods A rat model of adjuvant arthritis was established and randomly divided into blank group, AA model group, methotrexate positive drug control group and artesunate large, medium and small dose groups (20.0,10.0,2.5 mg.kg .d-1), and intragastric administration. The effects of monocyte chemotactic factor (MCP-1), RANTES and tumor necrosis factor (TNF-α) on activated T cells were detected by ELISA. Results Compared with the model group, artesunate small, medium and low dose groups could significantly reduce the level of serum TNF-α (P <0.05); artesunate low dose group and its large and medium dose groups Methotrexate group was significantly different (P <0.05); artesunate dose group can significantly reduce the expression of MCP-1 and RANTES in serum, compared with the model group, the difference was statistically significant (P < 0.05). However, there was a significant difference between the low-dose artesunate group and the large-dose and medium-dose groups or the methotrexate group (P <0.05). Conclusion Artesunate anti-inflammatory and immunomodulatory effects may be related to the inhibition of inflammatory cytokines.