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以十六烷基胺与十六烷基溴为原料,通过取代反应与缩合反应等5步反应分别合成人工脂质(肽脂质)与人工受体。利用自组装技术在脂质体上构建了超分子开关体系,其中磷酸吡哆醛(PLP)为信号分子,铜离子做为中间信使。在没有调控信号时,铜离子与乳酸脱氢酶结合,抑制酶的活性;当加入信号分子PLP时,信号分子与人工受体作用形成席夫碱,席夫碱与乳酸脱氢酶竞争铜离子,解除铜离子对乳酸脱氢酶活性的抑制,恢复乳酸脱氢酶的催化活性。
Cetyl amine and cetyl bromide were used as starting materials to synthesize artificial lipids (peptide lipids) and artificial receptors respectively by five steps of substitution reaction and condensation reaction. The self-assembly technique was used to construct a supramolecular switch system on liposomes, in which pyridoxal phosphate (PLP) is a signaling molecule and copper ion as an intermediate messenger. In the absence of regulatory signals, copper ions combine with lactate dehydrogenase to inhibit the activity of the enzyme. When the signal molecule PLP is added, the signal molecule acts as an artificial receptor to form a Schiff base and the Schiff base competes for copper ion with lactate dehydrogenase , Release of copper ions on the inhibition of lactate dehydrogenase activity, restore the catalytic activity of lactate dehydrogenase.