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目的：研究制备抗皂角毒和表皮生长因子受体双功能抗体，用以导向皂角毒杀伤肿瘤细胞。方法：在制备ＥＱ７５、ＣＹ１２．１４单克隆抗体细胞株的基础上，通过杂交杂交瘤技术制备双功能抗体细胞株。结果：双功能抗体（ＥＱ７５×ＣＹ１２．１４）与单用皂角毒杀伤肿瘤细胞（Ａ４３１）的ＩＣ５０分别为１０－９．６、１０－８．４ｍｏｌ／Ｌ。结论：双功能抗体（ＥＱ７５×ＣＹ１２．１４）杀伤肿瘤细胞（Ａ４３１），以ＩＣ５０值计算，有效率为单用皂角毒的１５倍左右。
OBJECTIVE: To prepare bifunctional antibodies against scutellariae gondii and epidermal growth factor receptors, which are used to direct cytotoxic effects of saponin. Methods: Based on the preparation of EQ75 and CY12.14 monoclonal antibody cell lines, bispecific antibody cell lines were prepared by hybrid hybridoma technology. RESULTS: The IC50 of bifunctional antibody (EQ75 x CY12.14) and killing of tumor cells (A431) with saponin alone was 10-9.6 and 10-8.4 mol/L, respectively. Conclusion: The dual function antibody (EQ75×CY12.14) kills the tumor cells (A431). The effective rate is calculated to be about 15 times that of saponin alone.